Generation of a CHIP isogenic human iPSCderived cortical neuron model for functional proteomics

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F22%3A00079118" target="_blank" >RIV/00209805:_____/22:00079118 - isvavai.cz</a>
Result on the web
<a href="https://pubmed.ncbi.nlm.nih.gov/35391935/" target="_blank" >https://pubmed.ncbi.nlm.nih.gov/35391935/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.xpro.2022.101247" target="_blank" >10.1016/j.xpro.2022.101247</a>

Result language
angličtina
Original language name
Generation of a CHIP isogenic human iPSCderived cortical neuron model for functional proteomics
Original language description
The neuroprotective E3-ubiquitin ligase CHIP is linked to healthy aging. Here, we present a protocol using a patient-derived iPSC line with a triplication of the a-synuclein gene to produce gene-edited cells isogenic for CHIP. We describe iPSC differentiation into cortical neurons and their identity validation. We then detail mass spectrometry-based approaches (SWATH-MS) to identify dominant changes in the steady state proteome generated by loss of CHIP function. This protocol can be adapted to other proteins that impact proteostasis in neurons.
Czech name
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Czech description
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Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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