Study of Protein Conformational Dynamics Using Hydrogen/Deuterium Exchange Mass Spectrometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F23%3A00079274" target="_blank" >RIV/00209805:_____/23:00079274 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/23:00133541
Result on the web
<a href="https://pubmed.ncbi.nlm.nih.gov/37093484/" target="_blank" >https://pubmed.ncbi.nlm.nih.gov/37093484/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-3147-8_18" target="_blank" >10.1007/978-1-0716-3147-8_18</a>
Alternative languages
Result language
angličtina
Original language name
Study of Protein Conformational Dynamics Using Hydrogen/Deuterium Exchange Mass Spectrometry
Original language description
Intrinsic protein dynamics contribute to their biological functions. Rational engineering of protein dynamics is extremely challenging with only a handful of successful examples. Hydrogen/deuterium exchange coupled to mass spectrometry (HDX-MS) represents a powerful technique for quantitative analysis of protein dynamics. Here we provide a detailed description of the preparation of protein samples, collection of high-quality data, and their in-depth analysis using various computational tools. We illustrate the application of HDX-MS for the study of protein dynamics in the rational engineering of flexible loops in the reconstructed ancestor of haloalkane dehalogenase and Renilla luciferase. These experiments provided unique and valuable data rigorously describing the modification of protein dynamics upon grafting of the loop-helix element. Tips and tricks are provided to stimulate the wider use of HDX-MS to study and engineer protein dynamics. (C) 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Advanced Methods in Structural Biology
ISBN
978-1-07-163146-1
Number of pages of the result
26
Pages from-to
1-26
Number of pages of the book
462
Publisher name
Humana Press
Place of publication
Totowa
UT code for WoS chapter
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