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Study of Protein Conformational Dynamics Using Hydrogen/Deuterium Exchange Mass Spectrometry

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F23%3A00079274" target="_blank" >RIV/00209805:_____/23:00079274 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14310/23:00133541

  • Result on the web

    <a href="https://pubmed.ncbi.nlm.nih.gov/37093484/" target="_blank" >https://pubmed.ncbi.nlm.nih.gov/37093484/</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/978-1-0716-3147-8_18" target="_blank" >10.1007/978-1-0716-3147-8_18</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Study of Protein Conformational Dynamics Using Hydrogen/Deuterium Exchange Mass Spectrometry

  • Original language description

    Intrinsic protein dynamics contribute to their biological functions. Rational engineering of protein dynamics is extremely challenging with only a handful of successful examples. Hydrogen/deuterium exchange coupled to mass spectrometry (HDX-MS) represents a powerful technique for quantitative analysis of protein dynamics. Here we provide a detailed description of the preparation of protein samples, collection of high-quality data, and their in-depth analysis using various computational tools. We illustrate the application of HDX-MS for the study of protein dynamics in the rational engineering of flexible loops in the reconstructed ancestor of haloalkane dehalogenase and Renilla luciferase. These experiments provided unique and valuable data rigorously describing the modification of protein dynamics upon grafting of the loop-helix element. Tips and tricks are provided to stimulate the wider use of HDX-MS to study and engineer protein dynamics. (C) 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

  • Czech name

  • Czech description

Classification

  • Type

    C - Chapter in a specialist book

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Book/collection name

    Advanced Methods in Structural Biology

  • ISBN

    978-1-07-163146-1

  • Number of pages of the result

    26

  • Pages from-to

    1-26

  • Number of pages of the book

    462

  • Publisher name

    Humana Press

  • Place of publication

    Totowa

  • UT code for WoS chapter