In-frame germline TP53 variant impairs p53 oligomerization and predisposes to cancer

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F25%3A00080386" target="_blank" >RIV/00209805:_____/25:00080386 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/25:00638564 RIV/00216224:14110/25:00141890 RIV/00216208:11110/25:10501192 RIV/65269705:_____/25:00082605 RIV/00064165:_____/25:10501192
Result on the web
<a href="https://www.nature.com/articles/s41598-025-14684-8" target="_blank" >https://www.nature.com/articles/s41598-025-14684-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-025-14684-8" target="_blank" >10.1038/s41598-025-14684-8</a>

Result language
angličtina
Original language name
In-frame germline TP53 variant impairs p53 oligomerization and predisposes to cancer
Original language description
Germline loss-of-function variants in TP53 cause Li-Fraumeni syndrome (LFS) characterized by an early onset of various cancer types including sarcomas, adrenocortical carcinoma, and breast cancer. The most common are mutations in the DNA binding domain of p53, but alterations in the oligomerization domain also cause LFS with variable level of penetrance. Here we report identification of a novel germline in-frame deletion TP53 variant c.1015_1023del p.(E339_F341del) in a family with early-onset breast cancer and other malignancies. Using functional testing, we found that a short deletion in the oligomerization domain in the p.E339_F341del variant severely impaired transcriptional activity of p53 in human cells and in a yeast model. The loss of the transactivation activity was consistent with an observed defect in formation of p53 tetramers. Finally, we found that cells expressing the p.E339_F341del variant were insensitive to inhibition of MDM2 by nutlin-3 confirming the functional defect. We conclude that the in-frame germline c.1015_1023del TP53 variant encodes a transcriptionally inactive protein and promotes LFS with a high penetrant cancer phenotype.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30204 - Oncology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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