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Cytokinin arabinosides show increased efficacy in priming tomato immunity and inhibiting the fungal phytopathogen B. cinerea

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F25%3A00080443" target="_blank" >RIV/00209805:_____/25:00080443 - isvavai.cz</a>

  • Alternative codes found

    RIV/61389030:_____/25:00641187 RIV/61989592:15310/25:73632016

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/pii/S2667064X25003239" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2667064X25003239</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.stress.2025.101055" target="_blank" >10.1016/j.stress.2025.101055</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Cytokinin arabinosides show increased efficacy in priming tomato immunity and inhibiting the fungal phytopathogen B. cinerea

  • Original language description

    Cytokinins (CKs) regulate numerous biological processes in plants, and are involved in plant growth and development as well as responses to biotic and abiotic stresses. Although CKs have been reported to prime plant immunity, thus far, research on CK hormopriming has focused on classical synthetic CKs such as kinetin or 6-benzylaminopurine (BAP). Here, we show that synthetic aromatic CK derivatives-specifically, 3-methoxy-6-benzylaminopurine-9-arabinosides-effectively induce immune responses and promote resistance to Botrytis cinerea in tomato (Solanum lycopersicum) primarily via a salicylic acid (SA)-dependent mechanism. Additionally, like 6- BAP, these compounds directly inhibit B. cinerea. Transcriptomic analyses of B. cinerea treated with CK arabinosides revealed significant changes in expressed gene profiles, notably in pathways associated with sugar metabolism. Unlike classical CKs, CK arabinosides do not alleviate growth inhibition under sugar-limited conditions, contributing to their stronger antifungal effects. Furthermore, CK arabinosides induce tomato immune responses more robustly than classical CKs, yet without activating canonical cytokinin signaling pathways, likely owing to their exceptional chemical stability which prevents release of the free active CK base, and due to their inability to directly bind CK receptors. Altogether, our findings suggest that CK arabinosides act via a dual mechanism-immune priming and direct pathogen inhibition-and hold promise as next-generation hormopriming agents for sustainable crop protection.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10611 - Plant sciences, botany

Result continuities

  • Project

    <a href="/en/project/EH22_008%2F0004581" target="_blank" >EH22_008/0004581: TowArds Next GENeration Crops (TANGENC)</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2025

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Plant stress

  • ISSN

    2667-064X

  • e-ISSN

    2667-064X

  • Volume of the periodical

    18

  • Issue of the periodical within the volume

    December 2025

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    12

  • Pages from-to

    101055

  • UT code for WoS article

    001594551300001

  • EID of the result in the Scopus database

    2-s2.0-105018178878