Circulating Serum piRNAs as Diagnostic and Prognostic Tools for Clear Cell Renal Cell Carcinoma

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F25%3A00080450" target="_blank" >RIV/00209805:_____/25:00080450 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/25:00142393 RIV/65269705:_____/25:00082937

Result on the web

<a href="https://cgp.iiarjournals.org/content/22/6/888.long" target="_blank" >https://cgp.iiarjournals.org/content/22/6/888.long</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.21873/cgp.20545" target="_blank" >10.21873/cgp.20545</a>

Result language

angličtina

Original language name

Circulating Serum piRNAs as Diagnostic and Prognostic Tools for Clear Cell Renal Cell Carcinoma

Original language description

BACKGROUND/AIM: The incidence of cancer continues to rise, highlighting the urgent need for reliable, non-invasive biomarkers to support early diagnosis and improve treatment outcomes. In addition to circulating microRNAs, circulating PIWI-interacting RNAs (cir-piRNAs) have emerged as promising candidates. Although the biological functions of piRNAs are not yet fully understood, they are known to suppress transposable elements and may regulate structural genes involved in tumorigenesis. In this two-phase study, we aimed to identify serum piRNAs with diagnostic potential for clear cell renal cell carcinoma (ccRCC). MATERIALS AND METHODS: A total of 238 serum samples from ccRCC patients and 208 healthy controls were analyzed. In the exploratory phase, next-generation sequencing (NGS) was performed on pooled samples representing different clinical stages of ccRCC and healthy controls. RESULTS: We identified 35 piRNAs with significantly different expression (p&lt;0.01) between groups. Based on statistical significance, read abundance, and fold change, six piRNAs were selected for the training phase and subsequently, three piRNAs, piR-24672, piR-27140, and piR-28876, were selected for validation. Validation by RT-qPCR in an independent cohort confirmed significantly reduced levels of all three piRNAs in ccRCC patients. ROC analysis demonstrated that piR-28876 is a superior diagnostic biomarker, reaching AUC=0.787, with 85.0% specificity and 66.3% sensitivity in distinguishing ccRCC patients from healthy controls. CONCLUSION: Circulating piRNAs, particularly piR-24672, piR-27140, and piR-28876, may serve as potential non-invasive biomarkers for the early detection of ccRCC.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30204 - Oncology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cancer genomics &amp; proteomics

ISSN

1109-6535

e-ISSN

1790-6245

Volume of the periodical

22

Issue of the periodical within the volume

6

Country of publishing house

GR - GREECE

Number of pages

12

Pages from-to

888-899

UT code for WoS article

001607415600005

EID of the result in the Scopus database

2-s2.0-105020356889