Reduced structural rigidity of MDMX protein enhances binding to TP53 mRNA

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F25%3A00080505" target="_blank" >RIV/00209805:_____/25:00080505 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/25:00642318 RIV/00216224:14310/25:00143440

Result on the web

<a href="https://portlandpress.com/bioscirep/article/45/11/BSR20253646/236760/Reduced-structural-rigidity-of-MDMX-protein" target="_blank" >https://portlandpress.com/bioscirep/article/45/11/BSR20253646/236760/Reduced-structural-rigidity-of-MDMX-protein</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1042/BSR20253646" target="_blank" >10.1042/BSR20253646</a>

Result language

angličtina

Original language name

Reduced structural rigidity of MDMX protein enhances binding to TP53 mRNA

Original language description

The two murine double minute (MDM) family members, MDM2 and MDMX are a well-established negative regulator of p53 activity. Under DNA damage conditions, MDM2 and MDMX are phosphorylated near their RING domains (serine 395 at MDM2 and serine 403 at MDMX) and switch to act as p53 positive regulators. MDMX binds to TP53 mRNA and acts as a chaperone for RNA structure, enabling MDM2 to bind. This interaction enhances TP53 mRNA translation, leading to increased p53 protein production. While the biological significance of this interaction has been described, the specific features of the MDMX-RNA interaction remain poorly understood. We used various MDMX protein constructs to characterize binding to TP53 mRNA and identified that the interaction mediated by the RING domain is modulated by the presence of other domains. Hydrogen-deuterium exchange mass spectrometry (HDX-MS) and binding assays in high salt conditions and various pH demonstrate that the whole protein participates in RNA interaction, with the C-terminal domain likely providing the contact with RNA by electrostatic forces. We show that protein structural changes induced by the chelating agent EDTA or the reducing agent TCEP enhances RNA binding by promoting partial structural destabilization of the protein. Our findings suggest that the MDMX/TP53 mRNA interaction is complex, with the RING domain binding to RNA and being supported by the entire protein, which acts as a scaffold for the RNA interaction. These results contribute to a better understanding of MDMX&apos;s role in TP53 mRNA binding and provide valuable insights for future investigation of the MDM2-MDMX-TP53 mRNA complex, which is crucial for p53 stabilization and activation under DNA-damaging conditions.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bioscience reports

ISSN

0144-8463

e-ISSN

1573-4935

Volume of the periodical

45

Issue of the periodical within the volume

11

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

"BSR20253646"

UT code for WoS article

001632690900001

EID of the result in the Scopus database

2-s2.0-105022815917