Semisynthesis of C17:0 isoforms of sulphatide and glucosylceramide using immobilised sphingolipid ceramide N-deacylase for application in analytical mass spectrometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F10%3A7698" target="_blank" >RIV/00216208:11110/10:7698 - isvavai.cz</a>

Alternative codes found

RIV/61389013:_____/10:00346664 RIV/00216275:25310/10:39882345

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Semisynthesis of C17:0 isoforms of sulphatide and glucosylceramide using immobilised sphingolipid ceramide N-deacylase for application in analytical mass spectrometry

Original language description

Immobilized sphingolipid ceramide N-deacylase (Pseudomonas sp. TK 4, EC 3.5.1.69), a hydrolytic enzyme with reverse synthetic activity was used for semisynthesis of C17:0 glucosylceramide and C17:0 sulphatide, which are required internal standards for tandem mass spectrometry. A high rate of conversion, reusability, long-term stability, and reduced contamination with other isoforms are advantages of this method. This immobilized enzyme system can be universally used for the simple preparation of specifically labelled sphingolipids of high isoform purity for application in mass spectrometry and in different fields of sphingolipid biochemistry.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA203%2F09%2F0857" target="_blank" >GA203/09/0857: New analytical approaches for identification of proteins with significant attributes for virulence and pathogenity of bacteria</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Rapid Communications in Mass Spectrometry

ISSN

0951-4198

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

16

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

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UT code for WoS article

000280687200011

EID of the result in the Scopus database

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