Comparison of Three Single Platform Methods for CD34+Hematopoietic Stem Cell Enumeration by Flow Cytometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F11%3A11293" target="_blank" >RIV/00216208:11110/11:11293 - isvavai.cz</a>

Alternative codes found

RIV/00064165:_____/11:11293 RIV/00023736:_____/11:00009520

Result on the web

<a href="http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS&DestLinkType=FullRecord&UT=000298897400026" target="_blank" >http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS&DestLinkType=FullRecord&UT=000298897400026</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Comparison of Three Single Platform Methods for CD34+Hematopoietic Stem Cell Enumeration by Flow Cytometry

Original language description

In the present study, we compared three single platform methods for CD34+ hematopoietic stem cell (HSC) enumeration by flow cytometry. For this purpose, we analyzed the performance characteristics and results obtained from different HSC sources. Interlaboratory coefficients of variation (CV) for precision/reproducibility analysis varied from 4.0% to 6.7% / 6.7% to 9.2% for the low and 3.2% to 4.1% / 4.3% to 6.7%, respectively, for the high stem cell control. Correlation between methods ranged from 0.92%to 0.99%; Wilcoxon test showed no significant differences (p >0.05); Bland-Altman analysis confirmed good agreement between assays (mean bias ranging from -0.48 to 6.91). Our results demonstrate very good intralaboratory correlation and agreement between methods, confirm the major impact of single platform strategy for accurate and reproducible HSC enumeration and suggest that high interlaboratory variability could be influenced by incorrect performance of validated methods.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

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Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Clinical Laboratory

ISSN

1433-6510

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

11

Country of publishing house

DE - GERMANY

Number of pages

5

Pages from-to

1031-1035

UT code for WoS article

000298897400026

EID of the result in the Scopus database

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