Phosphoprotein electrophoresis in the presence of Fe(III) ions

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F11%3A8873" target="_blank" >RIV/00216208:11110/11:8873 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/11:10107514
Result on the web
<a href="http://dx.doi.org/10.1002/jssc.201100321" target="_blank" >http://dx.doi.org/10.1002/jssc.201100321</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Phosphoprotein electrophoresis in the presence of Fe(III) ions
Original language description
Preparation of affinity polyacrylamide gels containing immobilized Fe(III) ions for the separation of proteins exhibiting metal ion binding properties is described. The presented method enables uniform distribution of immobilized metal ions in the affinity part of the polyacrylamide separating gel. Affinity gels prepared by this way are suitable to follow the effect of different concentrations of metal ions immobilized in polyacrylamide gel on a protein electrophoretic behavior. Polyacrylamide gels containing immobilized Fe(l I I) ions were used to study the electrophoretic behavior of two model proteins differing in their phosphate group content: chicken ovalbumin and bovine a-casein. For the electrophoretic separation, both the native and the denaturating conditions were used.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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