Comparison of the IHC, FISH, SISH and qPCR methods for the molecular diagnosis of breast cancer

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F12%3A13463" target="_blank" >RIV/00216208:11110/12:13463 - isvavai.cz</a>

Alternative codes found

RIV/00064165:_____/12:13463

Result on the web

<a href="http://dx.doi.org/10.3892/mmr.2012.919" target="_blank" >http://dx.doi.org/10.3892/mmr.2012.919</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Comparison of the IHC, FISH, SISH and qPCR methods for the molecular diagnosis of breast cancer

Original language description

Her2 proto-oncogene amplification and protein overexpression is observed in 20-40% of patients with breast cancer and plays a crucial role in invasive breast cancer and its treatment. In the present study, we investigated samples from 131 patients with invasive breast carcinoma. In all cases, the overexpression/amplification level of Her2 was determined using manual immunohistochemistry (IHC) and/or automatic IHC, fluorescence in situ hybridization (FISH), silver in situ hybridization (SISH) and quantitative polymerase chain reaction (qPCR). Using various methods, we demonstrated candidate methods for Her2 detection and their dependability. Our results demonstrate that these methods are highly comparable for the detection of Her2 overexpression/amplification. It was also revealed that qPCR is a valuable tool for the evaluation of Her2 gene overexpression/amplification. The results from pPCR analysis positively correlated with the results from IHC and FISH analysis. Moreover, in contras

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FP - Other medical fields

OECD FORD branch

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Project

<a href="/en/project/NS10575" target="_blank" >NS10575: Study of the mechanism of neoadjuvant chemotherapy-induced cell death and mechanism of chemotherapy resistance in patient with breast carcinoma</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

MOLECULAR MEDICINE REPORTS

ISSN

1791-2997

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

2

Country of publishing house

GR - GREECE

Number of pages

5

Pages from-to

439-443

UT code for WoS article

000305927700033

EID of the result in the Scopus database

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