Analysis of human blood plasma and hen egg white by chiroptical spectroscopic methods (ECD, VCD, ROA)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F13%3A10196294" target="_blank" >RIV/00216208:11110/13:10196294 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22340/13:43895054 RIV/00064165:_____/13:10196294

Result on the web

<a href="http://dx.doi.org/10.1007/s00216-013-6946-6" target="_blank" >http://dx.doi.org/10.1007/s00216-013-6946-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-013-6946-6" target="_blank" >10.1007/s00216-013-6946-6</a>

Result language

angličtina

Original language name

Analysis of human blood plasma and hen egg white by chiroptical spectroscopic methods (ECD, VCD, ROA)

Original language description

Chiroptical methods are widely used in structural and conformational analyses of biopolymers. The application of these methods to investigations of biofluids would provide new avenues for the molecular diagnosis of protein-misfolding diseases. In this work, samples of human blood plasma and hen egg white were analyzed using a combination of conventional and chiroptical methods: ultraviolet absorption/electronic circular dichroism (UV/ECD), Fourier transform infrared absorption/vibrational circular dichroism (FTIR/VCD), and Raman scattering/Raman optical activity (Raman/ROA). For comparison, the main components of these substances-human serum albumin (HSA) and ovalbumin (Ova)-were also analyzed by these methods. The ultraviolet region of the ECD spectrum was analyzed using the CDNN CD software package to evaluate the secondary structures of the proteins. The UV/ECD, FTIR/VCD, and Raman/ROA spectra of the substances were quite similar to those of the corresponding major proteins, while s

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

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Volume of the periodical

405

Issue of the periodical within the volume

16

Country of publishing house

DE - GERMANY

Number of pages

13

Pages from-to

5441-5453

UT code for WoS article

000319745700009

EID of the result in the Scopus database

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