Toxicity of carboxylated carbon nanotubes in endothelial cells is attenuated by stimulation of the autophagic flux with the release of nanomaterial in autophagic vesicles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10272443" target="_blank" >RIV/00216208:11110/14:10272443 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/14:00429962
Result on the web
<a href="http://dx.doi.org/10.1016/j.nano.2014.02.001" target="_blank" >http://dx.doi.org/10.1016/j.nano.2014.02.001</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.nano.2014.02.001" target="_blank" >10.1016/j.nano.2014.02.001</a>
Alternative languages
Result language
angličtina
Original language name
Toxicity of carboxylated carbon nanotubes in endothelial cells is attenuated by stimulation of the autophagic flux with the release of nanomaterial in autophagic vesicles
Original language description
Carbon nanotubes (CNTs) exhibit a number of unique properties that make them attractive for various nanomedicine applications including their intravascular use. Therefore, the vascular toxicity of CNTs is a critical safety concern and methods of CNTs toxicity modulation are of great interest. Here, we report that carboxylated multiwalled carbon nanotubes (MWCNTs) induce a decrease in viability of cultured human umbilical vein endothelial cells (HUVECs) associated with the profound accumulation of autophagosomes. This autophagosome accumulation was mTOR kinase independent and was caused by blockade of the autophagic flux rather than by activation of autophagy. Stimulation of the autophagic flux with 1 nmol/L bafilomycin A1 attenuated the cytotoxicity ofcarboxylated MWCNTs in HUVECs and was associated with the extracellular release of the nanomaterial in autophagic microvesicles. Thus, pharmacological stimulation of the autophagic flux may represent a new method of cytoprotection agains
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FP - Other medical fields
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LH12014" target="_blank" >LH12014: Evaluation of cellular toxicity of nanoparticles and protein amyloid fibrils.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nanomedicine: Nanotechnology, Biology, and Medicine
ISSN
1549-9634
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
5
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
939-948
UT code for WoS article
000338736200007
EID of the result in the Scopus database
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