Tracking DNA and RNA Sequences at High Resolution
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10284235" target="_blank" >RIV/00216208:11110/14:10284235 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/14:33149229
Result on the web
<a href="http://link.springer.com/protocol/10.1007%2F978-1-62703-776-1_16" target="_blank" >http://link.springer.com/protocol/10.1007%2F978-1-62703-776-1_16</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-62703-776-1_16" target="_blank" >10.1007/978-1-62703-776-1_16</a>
Alternative languages
Result language
angličtina
Original language name
Tracking DNA and RNA Sequences at High Resolution
Original language description
In this chapter, the basic principles and protocols of the electron microscopical detections of specific DNA and RNA sequences are described. We focused primarily on a comparison of various methods of electron microscopy in situ hybridization (EM-ISH) with respect to their sensitivity and the structural preservation of the sample with the aim of helping the readers select the appropriate hybridization protocol. As the post-embedding EM-ISH most frequently represents the optimal choice, the protocol forthe post-embedding EM-ISH approach is described in detail. Concurrently, the alternative methods based on the enzymatic synthesis of the labeled nucleic acids chains that can be used for the detection of DNA or RNA molecules in situ are mentioned. In this respect, the technique enabling the enzymatic detection of the polyadenylated RNA sequences is described in detail.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
EA - Morphology and cytology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Electron Microscopy; Methods and Protocols : Methods and Protocols
ISBN
978-1-62703-775-4
Number of pages of the result
24
Pages from-to
343-366
Number of pages of the book
799
Publisher name
Humana Press
Place of publication
New York
UT code for WoS chapter
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