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Tracking DNA and RNA Sequences at High Resolution

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10284235" target="_blank" >RIV/00216208:11110/14:10284235 - isvavai.cz</a>

  • Alternative codes found

    RIV/61989592:15110/14:33149229

  • Result on the web

    <a href="http://link.springer.com/protocol/10.1007%2F978-1-62703-776-1_16" target="_blank" >http://link.springer.com/protocol/10.1007%2F978-1-62703-776-1_16</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/978-1-62703-776-1_16" target="_blank" >10.1007/978-1-62703-776-1_16</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Tracking DNA and RNA Sequences at High Resolution

  • Original language description

    In this chapter, the basic principles and protocols of the electron microscopical detections of specific DNA and RNA sequences are described. We focused primarily on a comparison of various methods of electron microscopy in situ hybridization (EM-ISH) with respect to their sensitivity and the structural preservation of the sample with the aim of helping the readers select the appropriate hybridization protocol. As the post-embedding EM-ISH most frequently represents the optimal choice, the protocol forthe post-embedding EM-ISH approach is described in detail. Concurrently, the alternative methods based on the enzymatic synthesis of the labeled nucleic acids chains that can be used for the detection of DNA or RNA molecules in situ are mentioned. In this respect, the technique enabling the enzymatic detection of the polyadenylated RNA sequences is described in detail.

  • Czech name

  • Czech description

Classification

  • Type

    C - Chapter in a specialist book

  • CEP classification

    EA - Morphology and cytology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Book/collection name

    Electron Microscopy; Methods and Protocols : Methods and Protocols

  • ISBN

    978-1-62703-775-4

  • Number of pages of the result

    24

  • Pages from-to

    343-366

  • Number of pages of the book

    799

  • Publisher name

    Humana Press

  • Place of publication

    New York

  • UT code for WoS chapter