Rapid cooling of the amniotic membrane as a model system for the vitrification of posterior corneal lamellae

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10285228" target="_blank" >RIV/00216208:11110/14:10285228 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s10561-013-9388-7" target="_blank" >http://dx.doi.org/10.1007/s10561-013-9388-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10561-013-9388-7" target="_blank" >10.1007/s10561-013-9388-7</a>

Result language
angličtina
Original language name
Rapid cooling of the amniotic membrane as a model system for the vitrification of posterior corneal lamellae
Original language description
To vitrify human amniotic membrane specimens so that the maximum of epithelial cells survives in order to develop a procedure for the eventual vitrification of posterior corneal lamellae without using cryoprotective agents. To assess different methods oftissue sample preparation preceding vitrification. In group 1, the amniotic membrane specimens were stretched on nitrocellulose support. In group 2, mechanical pressure was used to remove the excess culture medium between the support and the membrane. The samples were frozen in liquid ethane (-183 A degrees C) and stored in liquid nitrogen. The specimens in the control group were not vitrified. Re-warming was performed at 40 A degrees C. The epithelial cell survival rate was assessed after 1, 3 and 7 days of storage following re-warming using calcein and ethidium homodimer-1 fluorescence. A wide range of values was observed among the different groups and among individual specimens within the groups. Resulting average survival rate was
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/GBP302%2F12%2FG157" target="_blank" >GBP302/12/G157: Dynamics and Organization of Chromosomes in the Cell Cycle and during Differentiation under Normal and Pathological Conditions</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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