Three-Dimensional Reconstruction of the S885A Mutant of Human Mitochondrial Lon Protease

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10285505" target="_blank" >RIV/00216208:11110/14:10285505 - isvavai.cz</a>

Result on the web

<a href="http://fb.cuni.cz/file/5747/fb2014a0034.pdf" target="_blank" >http://fb.cuni.cz/file/5747/fb2014a0034.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Three-Dimensional Reconstruction of the S885A Mutant of Human Mitochondrial Lon Protease

Original language description

The Lon protein is a protease belonging to the superfamily of ATPases associated with diverse cellular activities (AAA+). Its main function is the control of protein quality and the maintenance of proteostasis by degradation of misfolded and damaged proteins, which occur in response to numerous stress conditions. It also participates in the regulation of levels of transcription factors that control pathogenesis, development and stress response. We focus our interest on the structure of human mitochondrial Lon (hLon) protease, whose altered expression levels are linked to some severe diseases such as epilepsy, myopathy, or lateral sclerosis. We present the first 3D structure of the ADP-bound human Lon S885A mutant obtained by electron microscopy as a result of preliminary negative staining studies. S885A appears as a hexameric ring of 120 angstrom diameter having 90 angstrom in height. Its resolution was estimated at 19 angstrom by the FSC = 0.5 criterion. This model is a primary step t

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EA - Morphology and cytology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Biologica

ISSN

0015-5500

e-ISSN

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Volume of the periodical

60

Issue of the periodical within the volume

S1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

4

Pages from-to

62-65

UT code for WoS article

000343275800010

EID of the result in the Scopus database

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