PCR diagnostic system in the treatment of prosthetic joint infections

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F15%3A10284820" target="_blank" >RIV/00216208:11110/15:10284820 - isvavai.cz</a>
Alternative codes found
RIV/00064203:_____/15:10284820
Result on the web
<a href="http://dx.doi.org/10.1007/s12223-014-0370-y" target="_blank" >http://dx.doi.org/10.1007/s12223-014-0370-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12223-014-0370-y" target="_blank" >10.1007/s12223-014-0370-y</a>

Result language
angličtina
Original language name
PCR diagnostic system in the treatment of prosthetic joint infections
Original language description
In our prospective study, we examined whether a multiplex PCR diagnostic method is suitable for the primary detection of pathogens. We also examined the possibility and sensitivity of detecting genes responsible for biofilm production and methicillin resistance. From 2007 to 2009, 94 patients were included in the study. A UNB (universal detection of 16S ribosomal bacterial DNA) and UNF (universal detection of pathogenic fungi) were used in the primary detection. A multiplex assay for biofilm production,methicillin resistance allowed us to distinguish between Gram positivity and negativity and to detect Staphylococci. From all the samples, the culture was positive in 53.2 % of cases, and by using the UNB method, we detected bacteria in 79.8 % of cases-the UNF detection of fungi was positive in 10.6 % of cases. In 75 % of positive findings, we detected a Gram-negative bacterium in 65.3 % of cases. In 47.2 % of Staphylococci detected, the ability to produce biofilm was confirmed. 61.1 %
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FI - Traumatology and orthopaedics
OECD FORD branch
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Project
<a href="/en/project/NT14218" target="_blank" >NT14218: Use of antimicrobial peptides to treatment of musculoskeletal infections with the help of local carriers used in orthopedics</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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