Isoelectric Focusing of Serum Apolipoprotein C-III as a Sensitive Screening Method for the Detection of O-glycosylation Disturbances
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F15%3A10295581" target="_blank" >RIV/00216208:11110/15:10295581 - isvavai.cz</a>
Alternative codes found
RIV/00064165:_____/15:10295581
Result on the web
<a href="http://pmr.lf1.cuni.cz/media/pdf/pmr_2015116020073.pdf" target="_blank" >http://pmr.lf1.cuni.cz/media/pdf/pmr_2015116020073.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.14712/23362936.2015.48" target="_blank" >10.14712/23362936.2015.48</a>
Alternative languages
Result language
angličtina
Original language name
Isoelectric Focusing of Serum Apolipoprotein C-III as a Sensitive Screening Method for the Detection of O-glycosylation Disturbances
Original language description
Apolipoprotein C-III (ApoC-III) is a glycoprotein carrying the most common O-linked glycan structure and is abundantly present in serum, what renders it a suitable marker for analysis of O-glycosylation abnormalities. Isoelectric focusing followed by a Western blot of ApoC-III, using PhastSystemTM Electrophoresis System (GE Healthcare), was introduced as a rather simple and rapid method for screening of certain subtypes of inherited glycosylation disorders. The study's aim was to establish this method in our laboratory, what included performing the analysis in a group of 170 healthy individuals to set the reference range of detected relative amounts of sialylated ApoC-III isoforms and to evaluate the gender- and age-dependent differences. A significantrelative increase of asialo-ApoC-III with growing age was found. Secondly, we examined serum from patients with selected metabolic disorders and detected minor O-glycosylation changes in diseases such as Prader-Willi syndrome, PGM1 (phos
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FG - Paediatrics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/NT12166" target="_blank" >NT12166: The study of the bases of congenital disorders of glycosylation</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Prague medical report
ISSN
1214-6994
e-ISSN
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Volume of the periodical
116
Issue of the periodical within the volume
2
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
14
Pages from-to
73-86
UT code for WoS article
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EID of the result in the Scopus database
2-s2.0-84938944596