Reductive carboxylation and 2-hydroxyglutarate formation by wild-type IDH2 in breast carcinoma cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F15%3A10296857" target="_blank" >RIV/00216208:11110/15:10296857 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/15:00446813
Result on the web
<a href="http://dx.doi.org/10.1016/j.biocel.2015.05.012" target="_blank" >http://dx.doi.org/10.1016/j.biocel.2015.05.012</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.biocel.2015.05.012" target="_blank" >10.1016/j.biocel.2015.05.012</a>

Result language
angličtina
Original language name
Reductive carboxylation and 2-hydroxyglutarate formation by wild-type IDH2 in breast carcinoma cells
Original language description
Mitochondrial NADPH-dependent isocitrate dehydrogenase, IDH2, and cytosolic IDH1, catalyze reductive carboxylation of 2-oxoglutarate. Both idh2 and idh1 monoallelic mutations are harbored in grade 2/3 gliomas, secondary glioblastomas and acute myeloid leukemia. Mutant IDH1/IDH2 enzymes were reported to form an oncometabolite R-2-hydroxyglutarate (2HG), further strengthening malignancy. We quantified CO2-dependent reductive carboxylation glutaminolysis (RCG) and CO2-independent 2HG production in HTB-126and MDA-MB-231 breast carcinoma cells by measuring C-13 incorporation from 1-C-13-glutamine into citrate, malate, and 2HG. For HTB-126 cells, C-13-citrate, C-13-malate, and C-13-2-hydroxyglutarate were enriched by 2-, 5-, and 15-fold at 5 mM glucose (2-,2.5-, and 13-fold at 25 mM glucose), respectively, after 6 h. Such enrichment decreased by 6% with IDH1 silencing, but by 30-50% upon IDH2 silencing while cell respiration and ATP levels rose up to 150%. Unlike 2HG production RCG decline
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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