Pharmacological activation of estrogen receptors-alpha and -beta differentially modulates keratinocyte differentiation with functional impact on wound healing
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F16%3A10324736" target="_blank" >RIV/00216208:11110/16:10324736 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.3892/ijmm.2015.2351" target="_blank" >http://dx.doi.org/10.3892/ijmm.2015.2351</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3892/ijmm.2015.2351" target="_blank" >10.3892/ijmm.2015.2351</a>
Alternative languages
Result language
angličtina
Original language name
Pharmacological activation of estrogen receptors-alpha and -beta differentially modulates keratinocyte differentiation with functional impact on wound healing
Original language description
Estrogen deprivation is considered responsible for many age-related processes, including poor wound healing. Guided by previous observations that estradiol accelerates re-epithelialization through estrogen receptor (ER)-beta, in the present study, we examined whether selective ER agonists [4,4 ',4 ''-(4-propyl [1H] pyrazole-1,3,5-triyl)-trisphenol (PPT), ER-alpha agonist; 2,3-bis(4-hydroxyphenyl)-propionitrile (DPN), ER-beta agonist] affect the expression of basic proliferation and differentiation markers (Ki-67, keratin-10,-14 and -19, galectin-1 and Sox-2) of keratinocytes using HaCaT cells. In parallel, ovariectomized rats were treated daily with an ER modulator, and wound tissue was removed 21 days after wounding and routinely processed for basic histological analysis. Our results revealed that the HaCaT keratinocytes expressed both ER-alpha and -beta, and thus are well-suited for studying the effects of ER agonists on epidermal regeneration. The activation of ER-alpha produced a protein expression pattern similar to that observed in the control culture, with a moderate expression of Ki-67 being observed. However, the activation of ER-beta led to an increase in cell proliferation and keratin-19 expression, as well as a decrease in galectin-1 expression. Fittingly, in rat wounds treated with the ER-beta agonist (DPN), epidermal regeneration was accelerated. In the present study, we provide information on the mechanisms through which estrogens affect the expression patterns of selected markers, thus modulating keratinocyte proliferation and differentiation; in addition, we demonstrate that the pharmacological activation of ER-alpha and -beta has a direct impact on wound healing.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED1.1.00%2F02.0109" target="_blank" >ED1.1.00/02.0109: Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Medicine
ISSN
1107-3756
e-ISSN
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Volume of the periodical
37
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
21-28
UT code for WoS article
000367867000003
EID of the result in the Scopus database
2-s2.0-84948755106