Quantification of ethosuximide in human serum by GC-MS after butyl chloroformate derivatization
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F16%3A10336907" target="_blank" >RIV/00216208:11110/16:10336907 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/16:10336907
Result on the web
<a href="http://web.natur.cuni.cz/analchem/isc/isc12.pdf" target="_blank" >http://web.natur.cuni.cz/analchem/isc/isc12.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Quantification of ethosuximide in human serum by GC-MS after butyl chloroformate derivatization
Original language description
A simple, sensitive and robust method for determination of anti-epileptic drug ethosuximide in human serum using GC-MS was developed and validated for clinical toxicology purposes. This method employs an emerging class of derivatization agents: alkyl chloro-formates allowing the efficient and rapid derivatization of both the amino and carboxylic groups of the tested antiepileptic drug within seconds. The derivatization protocol was optimized using the Design of Experiment, and the entire sample preparation requires less than 5 minutes. Linear calibration curves were obtained in the concentration range 0.5-150 mu g/mL, with adequate accuracy (98.0-109.0%) and precision (< 10.4%). The method was successfully applied to quantification of ethosuximide in the serum of patients in both therapeutic and toxic concentration ranges
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
PROCEEDINGS OF THE 12TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY
ISBN
978-80-7444-044-1
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
17-22
Publisher name
CHARLES UNIV, FAC SCIENCE
Place of publication
PRAGUE 2
Event location
Prague
Event date
Sep 22, 2016
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000391253800004