Using corneal confocal microscopy to track changes in the corneal layers of dry eye patients after autologous serum treatment

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10332035" target="_blank" >RIV/00216208:11110/17:10332035 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/17:10332035 RIV/00216208:11150/17:10332035 RIV/00179906:_____/17:10332035 RIV/00064203:_____/17:10332035 and 2 more

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1111/cxo.12455/epdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/cxo.12455/epdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/cxo.12455" target="_blank" >10.1111/cxo.12455</a>

Result language

angličtina

Original language name

Using corneal confocal microscopy to track changes in the corneal layers of dry eye patients after autologous serum treatment

Original language description

Backround: In vivo corneal confocal microscopy allows the examination of each layer of the cornea in detail and the identification of pathological changes at the cellular level. The purpose of this study was to identify the possible effects of a three-month treatment with autologous serum eye-drops in different corneal layers of patients with severe dry eye disease using corneal confocal microscopy. Methods: Twenty-six patients with dry eye disease were included in the study. Corneal fluorescein staining was performed. The corneas of the right eyes were examined using in vivo corneal confocal microscopy before and after a three-month treatment with autologous serum drops. The densities of superficial and basal epithelial cells, Langerhans cells, the keratocytes and activated keratocytes, the density of endothelial cells and the status of the sub-basal nerve plexus fibres were evaluated. Results: A significant decrease in corneal fluorescein staining was found after the three-month autologous serum treatment (p = 0.0006). The basal epithelial cell density decreased significantly (p = 0.001), while the density of superficial epithelial cells did not change significantly (p = 0.473) nor did the number of Langerhans cells or activated keratocytes (p = 0.223; p = 0.307, respectively). There were no differences in the other corneal cell layers or in the status of the nerve fibres. Conclusions: The results demonstrate the ability of corneal confocal microscopy to evaluate an improvement in the basal epithelial cell layer of the cornea after autologous serum treatment in patients with dry eye disease. More studies with longer follow-up periods are needed to elucidate the suitability of corneal confocal microscopy to follow the effect of autologous serum treatment on nerve fibres or other corneal layers in dry eye disease patients.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30207 - Ophthalmology

Project

<a href="/en/project/7F14156" target="_blank" >7F14156: Tissue engineering of genetically competent corneal and conjunctival cells for subsequent grafting in human medicine</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Clinical and Experimental Optometry

ISSN

0816-4622

e-ISSN

—

Volume of the periodical

100

Issue of the periodical within the volume

3

Country of publishing house

AU - AUSTRALIA

Number of pages

7

Pages from-to

243-249

UT code for WoS article

000400290500007

EID of the result in the Scopus database

2-s2.0-85018714794