Cryopreserved human aortic root allografts arterial wall: Structural changes occurring during thawing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10360601" target="_blank" >RIV/00216208:11110/17:10360601 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/17:10360601 RIV/00216208:11150/17:10360601 RIV/00179906:_____/17:10360601 RIV/00064203:_____/17:10360601 RIV/00064165:_____/17:10360601

Result on the web

<a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0175007" target="_blank" >http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0175007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0175007" target="_blank" >10.1371/journal.pone.0175007</a>

Result language

angličtina

Original language name

Cryopreserved human aortic root allografts arterial wall: Structural changes occurring during thawing

Original language description

Background The aim of our experimental work was to assess morphological changes of arterial wall that arise during different thawing protocols of a cryopreserved human aortic root allograft (CHARA) arterial wall. Methods The experiment was performed on CHARAs. Two thawing protocols were tested: 1, CHARAs were thawed at a room temperature at + 23 degrees C; 2, CHARAs were placed directly into a water bath at + 37 degrees C. Microscopic samples preparation After fixation, all samples were washed in distilled water for 5 min, and dehydrated in a graded ethanol series ( 70, 85, 95, and 100%) for 5 min at each level. The tissue samples were then immersed in 100% hexamethyldisilazane for 10 minutes and air dried in an exhaust hood at room temperature. Processed samples were mounted on stainless steel stubs, coated with gold. Results Thawing protocol 1: All 6 ( 100%) samples showed loss of the endothelium and damage to the subendothelial layers with randomly dispersed circular defects and micro-fractures without smooth muscle cells contractions in the tunica media. Thawing protocol 2: All 6 (100%) samples showed loss of endothelium from the luminal surface, longitudinal corrugations in the direction of blood flow caused by smooth muscle cells contractions in the tunica media with frequent fractures in the subendothelial layer Conclusion All the samples thawed at the room temperature showed smaller structural damage to the CHARA arterial wall with no smooth muscle cell contraction in tunica media when compared to the samples thawed in a water bath.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30201 - Cardiac and Cardiovascular systems

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS One

ISSN

1932-6203

e-ISSN

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Volume of the periodical

12

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

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UT code for WoS article

000399874800013

EID of the result in the Scopus database

2-s2.0-85017530211