Initial cell adhesion of three cell types in the presence and absence of serum proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10361422" target="_blank" >RIV/00216208:11110/17:10361422 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11140/17:10361422 RIV/00216208:11310/17:10361422

Result on the web

<a href="https://link.springer.com/article/10.1007/s00418-017-1571-7" target="_blank" >https://link.springer.com/article/10.1007/s00418-017-1571-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00418-017-1571-7" target="_blank" >10.1007/s00418-017-1571-7</a>

Result language

angličtina

Original language name

Initial cell adhesion of three cell types in the presence and absence of serum proteins

Original language description

With the development of a wide range of new biomaterials for the sensing of different cell behaviour, it is important to consider whether the cells tested in vitro are in direct contact with the material or whether cell- biomaterial contact is mediated by an interfacial layer of proteins originating from the culture medium or from the cells themselves. Thus, this study describes the differences between the cell adhesion mediated by proteins originating from foetal bovine serum and without the presence of such proteins 2 h following cell seeding exemplarily with different cell types (an osteoblastic cell line, primary fibroblasts, and mesenchymal stem cells). Three of the examined cell types were found to react differently to differing conditions in terms of cell shape, area, and number. Nevertheless, the expression and localization of the various proteins involved in cell adhesion and signalling (CD44, vinculin, talin, actin, focal adhesion kinase, Rho-GTPases and extracellular signal- regulated kinases 1 and 2) were, in general, similar with respect to all the cell types tested, albeit varying according to the presence or absence of serum. Moreover, no classical focal adhesions were formed during cell adhesion without serum proteins, while different signalling pathways were involved in this process. The study systematically describes and discusses the cell adhesion of three different human cell types to a well-known substrate without the presence of external proteins and it is hoped that this knowledge will be subsequently applied in biomaterial applications in which the presence of external proteins is undesirable (e.g. for biosensing purposes).

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30106 - Anatomy and morphology (plant science to be 1.6)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Histochemistry and Cell Biology

ISSN

0948-6143

e-ISSN

—

Volume of the periodical

148

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

16

Pages from-to

273-288

UT code for WoS article

000407050400006

EID of the result in the Scopus database

2-s2.0-85018837651