Integral membrane proteins in proteomics. How to break open the black box?
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10361858" target="_blank" >RIV/00216208:11110/17:10361858 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.jprot.2016.08.006" target="_blank" >http://dx.doi.org/10.1016/j.jprot.2016.08.006</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jprot.2016.08.006" target="_blank" >10.1016/j.jprot.2016.08.006</a>
Alternative languages
Result language
angličtina
Original language name
Integral membrane proteins in proteomics. How to break open the black box?
Original language description
Integral membrane proteins (IMPs) are coded by 20-30% of human genes and execute important functions transmembrane transport, signal transduction, cell-cell communication, cell adhesion to the extracellular matrix, and many other processes. Due to their hydrophobicity, low expression and lack of trypsin cleavage sites in their transmembrane segments, IMPs have been generally under-represented in routine proteomic analyses. However, the field of membrane proteomics has changed markedly in the past decade, namely due to the introduction of filter assisted sample preparation (FASP), the establishment of cell surface capture (CSC) protocols, and the development of methods that enable analysis of the hydrophobic transmembrane segments. This review will summarize the recent developments in the field and outline the most successful strategies for the analysis of integral membrane proteins. Significance: Integral membrane proteins (IMPs) are attractive therapeutic targets mostly due to their many important functions. However, our knowledge of the membrane proteome is severely limited to effectively exploit their potential. This is mostly due to the lack of appropriate techniques or methods compatible with the typical features of IMPs, namely hydrophobicity, low expression and lack of trypsin cleavage sites. This review summarizes the most recent development in membrane proteomics and outlines the most successful strategies for their large-scale analysis.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Proteomics
ISSN
1874-3919
e-ISSN
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Volume of the periodical
153
Issue of the periodical within the volume
February
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
13
Pages from-to
8-20
UT code for WoS article
000393529100003
EID of the result in the Scopus database
2-s2.0-84995911618