Comparison of DNA isolation using salting-out procedure and automated isolation (MagNA system)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10362277" target="_blank" >RIV/00216208:11110/17:10362277 - isvavai.cz</a>
Alternative codes found
RIV/00064165:_____/17:10362277
Result on the web
<a href="http://dx.doi.org/10.1080/10826068.2017.1303613" target="_blank" >http://dx.doi.org/10.1080/10826068.2017.1303613</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/10826068.2017.1303613" target="_blank" >10.1080/10826068.2017.1303613</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of DNA isolation using salting-out procedure and automated isolation (MagNA system)
Original language description
Isolation of genomic DNA is a key step in genetic analysis. The aim of the study was to evaluate the suitability of isolation of DNA from peripheral blood with manual salting-out procedure and automated MagNA system under specific conditions. The impact of storage conditions, type of material (whole blood or blood cells), and method used for DNA extraction were evaluated in terms of DNA yield, its purity, and integrity. Fresh material, and material stored at 2-8 degrees C for 1-4 weeks and frozen at -80 degrees C were tested. For fresh samples, salting-out method gives higher yield than MagNA, irrespectively, on material used. Neither the yield of salting-out method nor its purity decreases during the storage of the samples in the fridge (2-8 degrees C) during 4 weeks. Concerning MagNA, storage of blood cells in the fridge decreases the yield of DNA as well as its purity. For frozen samples, for whole blood, MagNA gives better results while for blood cells, salting-out method seems to be better. For fresh samples, salting-out method is the preferred one, and both whole blood and blood cells can be used. For frozen samples, the preferred method depends on the material.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LM2015089" target="_blank" >LM2015089: Bank of Clinical Specimens</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Preparative Biochemistry and Biotechnology
ISSN
1082-6068
e-ISSN
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Volume of the periodical
47
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
703-708
UT code for WoS article
000407899900009
EID of the result in the Scopus database
2-s2.0-85017099093