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Comparison of DNA isolation using salting-out procedure and automated isolation (MagNA system)

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10362277" target="_blank" >RIV/00216208:11110/17:10362277 - isvavai.cz</a>

  • Alternative codes found

    RIV/00064165:_____/17:10362277

  • Result on the web

    <a href="http://dx.doi.org/10.1080/10826068.2017.1303613" target="_blank" >http://dx.doi.org/10.1080/10826068.2017.1303613</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1080/10826068.2017.1303613" target="_blank" >10.1080/10826068.2017.1303613</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Comparison of DNA isolation using salting-out procedure and automated isolation (MagNA system)

  • Original language description

    Isolation of genomic DNA is a key step in genetic analysis. The aim of the study was to evaluate the suitability of isolation of DNA from peripheral blood with manual salting-out procedure and automated MagNA system under specific conditions. The impact of storage conditions, type of material (whole blood or blood cells), and method used for DNA extraction were evaluated in terms of DNA yield, its purity, and integrity. Fresh material, and material stored at 2-8 degrees C for 1-4 weeks and frozen at -80 degrees C were tested. For fresh samples, salting-out method gives higher yield than MagNA, irrespectively, on material used. Neither the yield of salting-out method nor its purity decreases during the storage of the samples in the fridge (2-8 degrees C) during 4 weeks. Concerning MagNA, storage of blood cells in the fridge decreases the yield of DNA as well as its purity. For frozen samples, for whole blood, MagNA gives better results while for blood cells, salting-out method seems to be better. For fresh samples, salting-out method is the preferred one, and both whole blood and blood cells can be used. For frozen samples, the preferred method depends on the material.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    <a href="/en/project/LM2015089" target="_blank" >LM2015089: Bank of Clinical Specimens</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Preparative Biochemistry and Biotechnology

  • ISSN

    1082-6068

  • e-ISSN

  • Volume of the periodical

    47

  • Issue of the periodical within the volume

    7

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    6

  • Pages from-to

    703-708

  • UT code for WoS article

    000407899900009

  • EID of the result in the Scopus database

    2-s2.0-85017099093