Brain iron accumulation in Wilson disease: a post mortem 7 Tesla MRI - histopathological study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10363078" target="_blank" >RIV/00216208:11110/17:10363078 - isvavai.cz</a>

Alternative codes found

RIV/00064165:_____/17:10363078

Result on the web

<a href="http://dx.doi.org/10.1111/nan.12341" target="_blank" >http://dx.doi.org/10.1111/nan.12341</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/nan.12341" target="_blank" >10.1111/nan.12341</a>

Result language

angličtina

Original language name

Brain iron accumulation in Wilson disease: a post mortem 7 Tesla MRI - histopathological study

Original language description

AimsIn Wilson disease (WD), T2/T2*-weighted (T2*w) MRI frequently shows hypointensity in the basal ganglia that is suggestive of paramagnetic deposits. It is currently unknown whether this hypointensity is related to copper or iron deposition. We examined the neuropathological correlates of this MRI pattern, particularly in relation to iron and copper concentrations. MethodsBrain slices from nine WD and six control cases were investigated using a 7T-MRI system. High-resolution T2*w images were acquired and R2* parametric maps were reconstructed using a multigradient recalled echo sequence. R2* was measured in the globus pallidus (GP) and the putamen. Corresponding histopathological sections containing the lentiform nucleus were examined using Turnbull iron staining, and double staining combining Turnbull with immunohistochemistry for macrophages or astrocytes. Quantitative densitometry of the iron staining as well as copper and iron concentrations were measured in the GP and putamen and correlated with R2* values. ResultsT2*w hypointensity in the GP and/or putamen was apparent in WD cases and R2* values correlated with quantitative densitometry of iron staining. In WD, iron and copper concentrations were increased in the putamen compared to controls. R2* was correlated with the iron concentration in the GP and putamen, whereas no correlation was observed for the copper concentration. Patients with more pronounced pathological severity in the putamen displayed increased iron concentration, which correlated with an elevated number of iron-containing macrophages. ConclusionsT2/T2*w hypointensity observed in vivo in the basal ganglia of WD patients is related to iron rather than copper deposits.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30103 - Neurosciences (including psychophysiology)

Project

<a href="/en/project/NV15-25602A" target="_blank" >NV15-25602A: Biomarkers of progression and treatment response in neurodegenerative disorders</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Neuropathology and Applied Neurobiology

ISSN

0305-1846

e-ISSN

—

Volume of the periodical

43

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

19

Pages from-to

514-532

UT code for WoS article

000410552900005

EID of the result in the Scopus database

2-s2.0-84994807311