Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10363163" target="_blank" >RIV/00216208:11110/17:10363163 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/17:10363163 RIV/00064165:_____/17:10363163

Result on the web

<a href="http://dx.doi.org/10.1038/s41598-017-02905-8" target="_blank" >http://dx.doi.org/10.1038/s41598-017-02905-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-017-02905-8" target="_blank" >10.1038/s41598-017-02905-8</a>

Result language

angličtina

Original language name

Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response

Original language description

Circulating cell-free DNA (cfDNA) may be involved in immune response regulation. We studied the variations in abundance of telomeric sequences in plasma and serum in young healthy volunteers and the ability of cfDNA contained in these samples to co-activate the TNF-alpha m RNA expression in monocytes. We performed qPCR to determine relative telomere length (T/S ratios) in plasma, serum and whole blood of 36 volunteers. Using paired samples of plasma and serum and DNase treatment, we analysed the contribution of cfDNA to the co-activation of TNF-alpha mRNA expression in THP1 monocytic cell line. We found significant differences between paired plasma and serum samples in relative T/S ratios (median 1.38 +/- 1.1 vs. 0.86 +/- 0.25, respectively) and in total amounts of cfDNA and in estimated total amounts of telomeres which were significantly higher in serum than in plasma. TNF-alpha mRNA expression in THP1 cells increased significantly after DNase treatment of all samples used for stimulation. The highest TNF-a mRNA expressions were observed after stimulation with DNase treated serum samples. Our results suggest that the different content of telomeric sequences in plasma and serum may contribute to the tuning of immune response. Further studies of this interesting phenomenon are needed.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10600 - Biological sciences

Project

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Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

June

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

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UT code for WoS article

000402508300002

EID of the result in the Scopus database

2-s2.0-85020163461