Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10364016" target="_blank" >RIV/00216208:11110/17:10364016 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/17:00095662 RIV/65269705:_____/17:00067513 RIV/00209805:_____/17:00077829 RIV/00064190:_____/17:N0000081

Result on the web

<a href="http://dx.doi.org/10.11613/BM.2017.043" target="_blank" >http://dx.doi.org/10.11613/BM.2017.043</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.11613/BM.2017.043" target="_blank" >10.11613/BM.2017.043</a>

Result language

angličtina

Original language name

Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma

Original language description

Introduction: Urinary microRNAs (miRNAs) are emerging as a clinically useful tool for early and non-invasive detection of various types of cancer. The aim of this study was to evaluate whether let-7 family miRNAs differ in their urinary concentrations between renal cell carcinoma (RCC) cases and healthy controls. Materials and methods: In the case-control study, 69 non-metastatic clear-cell RCC patients and 36 gender/age-matched healthy controls were prospectively enrolled. Total RNA was purified from cell-free supernatant of the 105 first morning urine specimens. Let-7 family miRNAs were determined in cell-free supernatant using quantitative miRNA real-time reverse-transcription PCR and absolute quantification approach. Results: Concentrations of all let-7 miRNAs (let-7a, let-7b, let-7c, let-7d, let-7e and let-7g) were significantly higher in urine samples obtained from RCC patients compared to healthy controls (P &lt; 0.001; P &lt; 0.001; P = 0.005; P = 0.006; P = 0.015 and P = 0.002, respectively). Subsequent ROC analysis has shown that let-7a concentration possesses good ability to differentiate between cases and controls with area under curve being 0.8307 (sensitivity 71%, specificity 81%). Conclusions: We have shown that let-7 miRNAs are abundant in the urine samples of patients with clear-cell RCC, and out of six let-7 family members, let-7a outperforms the others and presents promising non-invasive biomarker for the detection of RCC.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30204 - Oncology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochemia Medica

ISSN

1330-0962

e-ISSN

—

Volume of the periodical

27

Issue of the periodical within the volume

2

Country of publishing house

HR - CROATIA

Number of pages

7

Pages from-to

411-417

UT code for WoS article

000404505700018

EID of the result in the Scopus database

2-s2.0-85020673994