Species-specific real-time RT-PCR analysis of expression of stromal cell genes in a tumor xenotransplantation model in mice
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10364402" target="_blank" >RIV/00216208:11110/17:10364402 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.bbrc.2017.07.061" target="_blank" >http://dx.doi.org/10.1016/j.bbrc.2017.07.061</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bbrc.2017.07.061" target="_blank" >10.1016/j.bbrc.2017.07.061</a>
Alternative languages
Result language
angličtina
Original language name
Species-specific real-time RT-PCR analysis of expression of stromal cell genes in a tumor xenotransplantation model in mice
Original language description
Human tumor xenografts in mice together with the species-specific analysis of expressed genes allow to study the molecular processes driving tumor growth and progression in vivo and help to develop and evaluate anticancer therapies. In the present work, we designed and validated species-specific real-time RT-PCR assays for discrimination and quantitation of expression of human and mouse transcripts in cancer and stromal cells including dipeptidyl peptidase (DPP) 4, DPP8, DPP9, fibroblast activation protein (FAP) and CXC chemokine receptor 4 in mixed human-mouse biological samples. Using single species RNA samples and mixed human-mouse RNA samples, we formulated and characterized two-step realtime RT-PCR assays to quantitate expression of the indicated transcripts and described analytical performance of the assays. We also demonstrated the applicability of these assays for species-specific quantitation of transcriptional expression of mouse stromal cell genes including Dpp4, DppS, Dpp9, Fap and Cxcr4 in mixed human-mouse RNA samples from human glioma cell-derived tumor xenografts growing in mouse brain.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30204 - Oncology
Result continuities
Project
<a href="/en/project/NV15-31379A" target="_blank" >NV15-31379A: Novel concepts for the therapeutic targeting of tumor microenvironment in human glioblastomas</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biochemical and Biophysical Research Communications
ISSN
0006-291X
e-ISSN
—
Volume of the periodical
491
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
126-133
UT code for WoS article
000408518700020
EID of the result in the Scopus database
2-s2.0-85023776003