Novel Approaches to Study Coronary Vasculature Development in Mice

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F18%3A10378062" target="_blank" >RIV/00216208:11110/18:10378062 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/18:00493023
Result on the web
<a href="https://doi.org/10.1002/DVDY.24637" target="_blank" >https://doi.org/10.1002/DVDY.24637</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/DVDY.24637" target="_blank" >10.1002/DVDY.24637</a>

Result language
angličtina
Original language name
Novel Approaches to Study Coronary Vasculature Development in Mice
Original language description
Background: Coronary artery development is an intensely studied field. Mice are a popular genetic model for developmental studies, but there is no widely accepted protocol for high-throughput, high-resolution imaging of their developmental and adult coronary artery anatomy. Results: Using tissue-clearing protocols and confocal microscopy, we have analyzed embryonic and juvenile mouse hearts in Cx40:GFP knock-in models with a special focus on septal artery development. We found that the septal artery, which supplies the interventricular septum, was initially formed as an arterial plexus that connected to both the left and right coronary arteries. During development, the plexus was remodeled into a single tube, which then remained connected only to the right coronary artery. Since optical imaging became limited at postnatal stages, it was supplemented with injection techniques using India ink and Microfil; the latter was subsequently analyzed with micro-CT to visualize the anatomy of coronary vessels in 3D. Conclusions: The techniques described here enable us to study the finer details of coronary artery development in mice and can, therefore, be implemented to study the pathogenesis of coronary malformations in various mouse models.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30106 - Anatomy and morphology (plant science to be 1.6)

Project
<a href="/en/project/GA13-12412S" target="_blank" >GA13-12412S: Development of mouse conduction system analyzed by 3D imaging and optical mapping</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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