The first multilocus genotype analysis of Giardia intestinalis in humans in the Czech Republic

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F18%3A10381664" target="_blank" >RIV/00216208:11110/18:10381664 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1017/S0031182018000409" target="_blank" >https://doi.org/10.1017/S0031182018000409</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1017/S0031182018000409" target="_blank" >10.1017/S0031182018000409</a>

Result language
angličtina
Original language name
The first multilocus genotype analysis of Giardia intestinalis in humans in the Czech Republic
Original language description
To date, genotyping data on giardiasis has not been available in the Czech Republic. In this study, we characterised 47 human isolates of Giardia intestinalis from symptomatic as well as asymptomatic giardiasis cases. Genomic DNA from trophozoites was tested by PCR-sequence analysis at three loci (β-giardin, glutamate dehydrogenase, and triose phosphate isomerase). Sequence analysis showed assemblages A and B in 41 (87.2%) and 6 (12.8%) isolates, respectively. Two of the 41 assemblage A samples were genotyped as sub-assemblage AI, and 39 were genotyped as sub-assemblage AII. Four previously identified multilocus genotypes (MLGs: AI-1, AII-1, AII-4 and AII-9) and six likely novel variations of MLGs were found. In agreement with previous studies, sequences from assemblage B isolates were characterised by larger genetic variability and by the presence of heterogeneous positions, which prevent the definition of MLGs. This study also investigated whether there was a relationship between the assemblage and clinical data (mainly drug resistance). However, due to the large number of genotypes and the relatively small number of samples, no significant correlations with the clinical data were found.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences

Project
<a href="/en/project/NV15-33369A" target="_blank" >NV15-33369A: Characteristics of isolates of Giardia intestinalis, a causative agent of the most common intestinal protozoan infection, as diagnostic tools</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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