Effect of smear layer deproteinization with chemo-mechanical caries removal agents on sealing performances of self-etch adhesives

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F20%3A10410441" target="_blank" >RIV/00216208:11110/20:10410441 - isvavai.cz</a>

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=cLxBze_LSh" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=cLxBze_LSh</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jdent.2020.103300" target="_blank" >10.1016/j.jdent.2020.103300</a>

Result language

angličtina

Original language name

Effect of smear layer deproteinization with chemo-mechanical caries removal agents on sealing performances of self-etch adhesives

Original language description

Objectives: To evaluate the sealing performance of self-etch adhesives (SEA) after smear layer deproteinization with chemo-mechanical caries removal agents (CRA) to enamel and dentin cavity walls in comparison with 6% NaOCl smear layer deproteinizing. Methods: Eighty extracted bovine incisors with tapered cylindrical cavities (2.5mm upper diameter, 1.5mm lower diameter, 2mm depth) at the cemento-enamel junction were randomly divided into four pre-treatment groups; no treatment (control), Papacarie (papain enzyme-based CRA), Carisolv (NaOCl-based CRA), and 6% NaOCl. After pretreatment, G-Bond Plus (GBP, one-step SEA) or Clearfil SE Bond 2 (CSE, two-step SEA) was applied to the pre-treated cavity, and then filled with a flowable resin composite (Clearfil Majesty ES Flow). Gap formations at the enamel and dentin lateral walls, and on the dentin cavity floor were investigated using swept-source optical coherence tomography (SS-OCT) after 24-h water storage at 37 degrees C. Deproteinizing effects of the tested agents on smear layer-covered enamel and dentin were quantified by changes in the amide:phosphate ratio using ATR-FTIR analysis. Results: Papacarie significantly reduced gap formation with both self-etch adhesives at all locations, compared to the control group (p &lt; 0.05). In contrast, increased gap formation was observed with Carisolv and NaOCl. In most groups, CSE exhibited significantly lower gap formation than GBP (p &lt; 0.05). ATR-FTIR revealed a significant decrease in the amide:phosphate ratio for all the deproteinizing agents (p &lt; 0.05). Conclusions: Chemo-mechanical CRAs could effectively remove the organic phase of enamel and dentin smear layer. The papain enzyme-based gel Papacarie could improve the sealing performance of the self-etch adhesives to both enamel and dentin.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30208 - Dentistry, oral surgery and medicine

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Dentistry

ISSN

0300-5712

e-ISSN

—

Volume of the periodical

94

Issue of the periodical within the volume

March

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

103300

UT code for WoS article

000518851100004

EID of the result in the Scopus database

2-s2.0-85079852989