High-Performance Liquid Chromatography as a Novel Method for the Determination of alpha-Defensins in Synovial Fluid for Diagnosis of Orthopedic Infections
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F20%3A10410841" target="_blank" >RIV/00216208:11110/20:10410841 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/20:10410841 RIV/00064203:_____/20:10410841
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=eQHJr7kw9G" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=eQHJr7kw9G</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/diagnostics10010033" target="_blank" >10.3390/diagnostics10010033</a>
Alternative languages
Result language
angličtina
Original language name
High-Performance Liquid Chromatography as a Novel Method for the Determination of alpha-Defensins in Synovial Fluid for Diagnosis of Orthopedic Infections
Original language description
The alpha-defensins (AD) present in synovial fluid have been regarded as constituting the most accurate periprosthetic joint infection (PJI) biomarker. The methods most commonly used for estimating AD as a biomarker are the qualitative Synovasure (R) PJI tests, based on the technique of lateral flow, and quantitative enzyme-linked immunosorbent assay (ELISA). Here, we propose a novel test based on detecting alpha-defensins in synovial fluid by high-performance liquid chromatography (HPLC). Synovial fluid was collected from 157 patients diagnosed with PJI, infectious arthritis (IA), arthrosis, reactive arthritis, and rheumatoid arthritis. AD concentrations in the fluid were determined by HPLC, and these same samples were used for additional diagnostic analyses. The results were statistically processed to calculate cutoff concentrations for PJI and IA. HPLC testing showed a sensitivity of 94% and a specificity of 92% for diagnosis of PJI, and a sensitivity of 97% and a specificity of 87% for diagnosis of IA. Using HPLC, we detected in synovial fluid a combination of three alpha-defensins: human neutrophil peptides HNP1, HNP2, and HNP3. All measured AD concentration values shown in this work refer to the sum of these three individual concentrations. Our study shows that the HPLC method meets the conditions for measuring precise concentrations of the sum of AD and can be recommended as part of a diagnostic array for PJI and IA diagnostics. By this method, we have verified that higher levels of AD in synovial fluid can also be seen in rheumatoid illnesses, crystal arthropathies, and reactive arthritis.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30211 - Orthopaedics
Result continuities
Project
<a href="/en/project/NV16-27726A" target="_blank" >NV16-27726A: Novel antimicrobial peptides for topical treatment of osteomyelitis and prevention of implant-related infections in orthopedics</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Diagnostics [online]
ISSN
2075-4418
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
1
Country of publishing house
CH - SWITZERLAND
Number of pages
10
Pages from-to
33
UT code for WoS article
000513129500037
EID of the result in the Scopus database
2-s2.0-85078444837