Direct tryptic cleavage in bone tissue followed by LC-MS/MS as a first step towards routine characterization of proteins embedded in alveolar bones
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F20%3A10412848" target="_blank" >RIV/00216208:11110/20:10412848 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/20:43921484 RIV/60461373:22340/20:43921484
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=0V-rAjjyTz" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=0V-rAjjyTz</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijms.2020.116375" target="_blank" >10.1016/j.ijms.2020.116375</a>
Alternative languages
Result language
angličtina
Original language name
Direct tryptic cleavage in bone tissue followed by LC-MS/MS as a first step towards routine characterization of proteins embedded in alveolar bones
Original language description
While methods like radiology or histology are routinely used in oral surgery, a simple and effective method for characterization of alveolar bones at the molecular level is missing. This is primarily due to the fact that proteomic analysis of hard tissue represents a serious analytical challenge. In this study, we developed a simple technique for the proteomic characterization of bone tissue in the field of oral surgery. After a prewashing step with different solvents, specific digestion with trypsin was performed directly in porcine alveolar bone tissues without the prior reduction and alkylation of disulfide bonds. The released peptides were then analyzed using liquid chromatography-tandem mass spectrometry. When 2-propanol was used in the pre-incubation step, this approach identified 487 proteins in the maxillary (upper jaw) bone and 443 proteins in the mandibular (lower jaw) bone, 40 of each being specifically ascribed to bone tissue. This suggests that routine use of our technique can provide deeper insights into the field of oral surgery at the molecular level. Furthermore, it could also have diagnostic potential in differentiating between the proteomic patterns of healthy and pathological alveolar bone tissue. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD018836.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LTAIN19007" target="_blank" >LTAIN19007: Development of Advanced Computational Algorithms for evaluating post-surgery rehabilitation</a><br>
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Mass Spectrometry
ISSN
1387-3806
e-ISSN
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Volume of the periodical
455
Issue of the periodical within the volume
September
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
116375
UT code for WoS article
000555567000008
EID of the result in the Scopus database
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