The Effects of Bilirubin and Lumirubin on Metabolic and Oxidative Stress Markers

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F21%3A10427888" target="_blank" >RIV/00216208:11110/21:10427888 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/21:43923114 RIV/00064165:_____/21:10427888

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=J.udbnx~hg" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=J.udbnx~hg</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fphar.2021.567001" target="_blank" >10.3389/fphar.2021.567001</a>

Result language

angličtina

Original language name

The Effects of Bilirubin and Lumirubin on Metabolic and Oxidative Stress Markers

Original language description

For severe unconjugated hyperbilirubinemia the gold standard treatment is phototherapy with blue-green light, producing more polar photo-oxidation products, believed to be non-toxic. The aim of the present study was to compare the effects of bilirubin (BR) and lumirubin (LR), the major BR photo-oxidation product, on metabolic and oxidative stress markers. The biological activities of these pigments were investigated on several human and murine cell lines, with the focus on mitochondrial respiration, substrate metabolism, reactive oxygen species production, and the overall effects on cell viability. Compared to BR, LR was found to be much less toxic, while still maintaining a similar antioxidant capacity in the serum as well as suppressing activity leading to mitochondrial superoxide production. Nevertheless, due to its lower lipophilicity, LR was less efficient in preventing lipoperoxidation. The cytotoxicity of BR was affected by the cellular glycolytic reserve, most compromised in human hepatoblastoma HepG2 cells. The observed effects were correlated with changes in the production of tricarboxylic acid cycle metabolites. Both BR and LR modulated expression of PPAR alpha downstream effectors involved in lipid and glucose metabolism. Proinflammatory effects of BR, evidenced by increased expression of TNF alpha upon exposure to bacterial lipopolysaccharide, were observed in murine macrophage-like RAW 264.7 cells. Collectively, these data point to the biological effects of BR and its photo-oxidation products, which might have clinical relevance in phototherapy-treated hyperbilirubinemic neonates and adult patients.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/NV18-07-00342" target="_blank" >NV18-07-00342: Studies of the bilirubin degradation products formed during phototherapy of neonatal jaundice</a><br>

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Pharmacology

ISSN

1663-9812

e-ISSN

—

Volume of the periodical

12

Issue of the periodical within the volume

March

Country of publishing house

CH - SWITZERLAND

Number of pages

18

Pages from-to

567001

UT code for WoS article

000629974300001

EID of the result in the Scopus database

2-s2.0-85102878617