Immunohistochemical analysis of selected stem cell markers including stage-specific mouse embryonic antigen 1 (SSEA-1) in porcine and bovine endometrium throughout the oestrous cycle
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F23%3A10466511" target="_blank" >RIV/00216208:11110/23:10466511 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11140/23:10466511 RIV/62157124:16170/23:43880775
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=3yAppTpIAi" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=3yAppTpIAi</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2754/avb202392030251" target="_blank" >10.2754/avb202392030251</a>
Alternative languages
Result language
angličtina
Original language name
Immunohistochemical analysis of selected stem cell markers including stage-specific mouse embryonic antigen 1 (SSEA-1) in porcine and bovine endometrium throughout the oestrous cycle
Original language description
There has been a lack of research regarding endometrial stem cells in farm animals, and particularly, regarding epithelial stem/progenitor cells. We aimed to identify potential stem cellsubpopulations in porcine and bovine endometrium by measuring the expression of selected stem cell markers (OCT3/4, CD44, SSEA-1, SOX10, CD73, and CD117) during the follicular and luteal phases of the oestrous cycle. We studied 28 endometrial tissue samples collected from 14 healthy, cycling pigs and cows. The endometrial mucosa of each sample was divided into basal, middle, and luminal portions, and the immunohistochemical staining intensity and percentages of cells that were marker-positive were recorded for each portion of the endometrium. Expression of OCT-3/4 was identified in the glands and stroma, and that of SOX10 and CD117 was identified in stroma of both porcine and bovine endometrium. In porcine endometrium, CD44 was only expressed in the glands, whereas SSEA-1 was expressed in the glands and stroma.In bovine endometrium, CD73 was only expressed in the glands. Differences in the expression of most of these markers were found between phases of the oestrous cycle and/or portions of the endometrial mucosa. Our data indicate the existence of both epithelial and mesenchymalstem cell subpopulations in the endometrium of pigs and cows during their oestrous cycles. The expression pattern of the stem cell marker SSEA-1 in porcine endometrium suggests a possible alternative location of the epithelial stem/progenitor cell population in the middle portion of theendometrial glands.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30106 - Anatomy and morphology (plant science to be 1.6)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Veterinaria Brno
ISSN
0001-7213
e-ISSN
1801-7576
Volume of the periodical
92
Issue of the periodical within the volume
3
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
8
Pages from-to
251-258
UT code for WoS article
001153359000004
EID of the result in the Scopus database
2-s2.0-85168787162