Large extracellular vesicles transfer more prions and infect cell culture better than small extracellular vesicles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F23%3A10472616" target="_blank" >RIV/00216208:11110/23:10472616 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/23:10472616
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=2v3Lr7aiz0" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=2v3Lr7aiz0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bbrc.2023.149208" target="_blank" >10.1016/j.bbrc.2023.149208</a>
Alternative languages
Result language
angličtina
Original language name
Large extracellular vesicles transfer more prions and infect cell culture better than small extracellular vesicles
Original language description
Prions are responsible for a number of lethal neurodegenerative and transmissible diseases in humans and animals. Extracellular vesicles, especially small exosomes, have been extensively studied in connection with various diseases. In contrast, larger microvesicles are often overlooked. In this work, we compared the ability of large extracellular vesicles (lEVs) and small extracellular vesicles (sEVs) to spread prions in cell culture. We utilized CAD5 cell culture model of prion infection and isolated lEVs by 20,000xg force and sEVs by 110,000xg force. The lEV fraction was enriched in beta-1 integrin with a vesicle size starting at 100 nm. The fraction of sEVs was partially depleted of beta-1 integrin with a mean size of 79 nm. Both fractions were enriched in prion protein, but the lEVs contained a higher prion-converting activity. In addition, lEV infection led to stronger prion signals in both cell cultures, as detected by cell and western blotting. These results were verified on N2a-PK1 cell culture. Our data suggest the importance of lEVs in the trafficking and spread of prions over extensively studied small EVs.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30303 - Infectious Diseases
Result continuities
Project
<a href="/en/project/LX22NPO5107" target="_blank" >LX22NPO5107: National institute for Neurological Research</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biochemical and Biophysical Research Communications
ISSN
0006-291X
e-ISSN
1090-2104
Volume of the periodical
687
Issue of the periodical within the volume
December
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
149208
UT code for WoS article
001112273600001
EID of the result in the Scopus database
2-s2.0-85176128201