A Fast HPLC/UV Method for Determination of Ketoprofen in Cellular Media

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F24%3A10471891" target="_blank" >RIV/00216208:11110/24:10471891 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22340/23:43928218 RIV/00064165:_____/24:10471891 RIV/60461373:22340/24:43928218
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=CeikzEViaL" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=CeikzEViaL</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/open.202300147" target="_blank" >10.1002/open.202300147</a>

Result language
angličtina
Original language name
A Fast HPLC/UV Method for Determination of Ketoprofen in Cellular Media
Original language description
A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media (EMEM, DMEM, RPMI). Separation was performed using a gradient on the C18 column with a mobile phase of acetonitrile and miliQ water acidified by 0.1 % (v/v) formic acid. The method was validated for parameters including linearity, accuracy, precision, limit of quantitation and limit of detection, as well as robustness. The response was found linear over the range of 3-100 mu g/mL as demonstrated by the acquired value of correlation coefficient R2=0.9997. The described method is applicable for determination of various pharmacokinetic aspects of ketoprofen in vitro. A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media. Separation was performed using a gradient on a C18 column with a mobile phase of acetonitrile and acidified miliQ water. The method was validated for linearity, accuracy, precision, limit of quantitation, limit of detection, as well as for robustness. The described method is applicable for determination of various pharmacokinetic aspects of ketoprofen in vitro.image
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30100 - Basic medicine

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)