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Artificial Intelligence (AI)-assisted readout method for the evaluation of skin prick automated test results

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F25%3A10504131" target="_blank" >RIV/00216208:11110/25:10504131 - isvavai.cz</a>

  • Result on the web

    <a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=th47zDtXpb" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=th47zDtXpb</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1038/s41467-025-64334-w" target="_blank" >10.1038/s41467-025-64334-w</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Artificial Intelligence (AI)-assisted readout method for the evaluation of skin prick automated test results

  • Original language description

    The skin prick test (SPT) is the gold standard for diagnosing allergic sensitization to aeroallergies. The Skin Prick Automated Test (SPAT) device has previously demonstrated reduced variability and more consistent test results compared to manual SPT. The current study aims to develop and validate an artificial intelligence (AI) assisted readout method to support physicians in interpreting skin reactions following SPAT. To train the AI algorithm, 7812 wheals (651 patients) are manually labeled. To validate the AI measurement, the longest wheal diameter of 2604 wheals (217 patients) is measured by the treating physician and compared to the AI measurement. In addition, AI-assisted readout is validated on a separate test cohort of 95 patients (1140 wheals). We demonstrate that the AI measurements of the longest wheal diameter exhibit a strong correlation with the physician&apos;s measurements. The AI algorithm shows a specificity of 98&lt;middle dot&gt;4% and sensitivity of 85&lt;middle dot&gt;0% in determining positive or negative test results in the validation cohort. In the test cohort, physicians adjust 5&lt;middle dot&gt;8% of AI measurements, leading to a change in the test interpretation for only 0&lt;middle dot&gt;5% of cases. AI-assisted readout significantly reduces inter- and intra-observer variability and readout time compared to manual physician measurements. Altogether, the AI-assisted readout method demonstrates high accuracy, with minimal misclassification of test results. Adding AI to SPAT further improves standardization across the SPT process, significantly reducing observer variability and time to readout.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30102 - Immunology

Result continuities

  • Project

  • Continuities

    V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Others

  • Publication year

    2025

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Nature Communications

  • ISSN

    2041-1723

  • e-ISSN

    2041-1723

  • Volume of the periodical

    16

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    8

  • Pages from-to

    8637

  • UT code for WoS article

    001586620700003

  • EID of the result in the Scopus database

    2-s2.0-105017806289