Two-dimensional electrophoretic comparison of metastatic and non-metastatic human breast tumors using in vitro cultured epithelial cells derived from the cancer tissues

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F08%3A00000520" target="_blank" >RIV/00216208:11120/08:00000520 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/08:2879 RIV/67985807:_____/08:00311358 RIV/68378050:_____/08:00311358 RIV/61388963:_____/08:00311358

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Two-dimensional electrophoretic comparison of metastatic and non-metastatic human breast tumors using in vitro cultured epithelial cells derived from the cancer tissues

Original language description

Background: Breast carcinomas represent a heterogeneous group of tumors diverse in behavior, outcome, and response to therapy. Identification of proteins resembling the tumor biology can improve the diagnosis, prediction, treatment selection, and targeting of therapy. Since the beginning of the post-genomic era, the focus of molecular biology gradually moved from genomes to proteins and proteomes and to their functionality. Proteomics can potentially capture dynamic changes in protein expression integrating both genetic and epigenetic influences. Methods: We prepared primary cultures of epithelial cells from 23 breast cancer tissue samples and performed comparative proteomic analysis. Seven patients developed distant metastases within three-year follow-up. These samples were included into a metastase-positive group, the others formed a metastase-negative group. Twodimensional electrophoretical (2-DE) gels in pH range 4?7 were prepared. Spot densities in 2-DE protein maps were subjected

Czech name

2-DE srovnání metastatických a nemetastatických lidských prsních nádorů při použití in-vitro kultivovaných epiteliálních buněk odvozených z nádorové tkáně

Czech description

Připravili jsme primární kultury epiteliálních buněk z 23 vzorků prsních karcinomů a provedli komparativní proteomickou analýzu. Byly připraveny 2-DE gely v rozmezí pH 4-7. Skvrny v 2-DE proteinové mapě byly statisticky analyzovány (R/maanova package a GUHA). Tři proteinové skvrny byly signifikantně odlišné u metastatických a nemetastatických skupin

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FP - Other medical fields

OECD FORD branch

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Project

<a href="/en/project/NR8323" target="_blank" >NR8323: Prognostic and predictive factors in breast cancer</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC Cancer

ISSN

1471-2407

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

107

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

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UT code for WoS article

000255938700001

EID of the result in the Scopus database

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