Examination of Specific DNA by PCR in Patients With Different Forms of Lyme Borreliosis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F08%3A00001164" target="_blank" >RIV/00216208:11120/08:00001164 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/08:4084

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Examination of Specific DNA by PCR in Patients With Different Forms of Lyme Borreliosis

Original language description

Background Borrelial specific DNA was examined in a group of 62 patients with different forms of Lyme borreliosis (LB) (32 patients suffered from neuroborreliosis, 19 manifested erythema migrans, and 11 joint involvement). Methods Nested-PCR system withfive newly derived primers was used in parallel. The study was organized prospectively, the presence of DNA was tested for plasma, CSF, joint fluid and urine before treatment, and plasma, joint fluid and urine were examined after treatment. Results Before therapy, 36 patients (58.1%) were DNA positive on the whole; 21 positive patients (65.6%) were found in the group of neuroborreliosis, 8 (42.1%) showed signs of skin involvement, and 7 (63.6%) were positive in arthritis. After treatment, 11 patients (36.7%) were positive in neuroborreliosis, 3 (17.6%) in skin form, and 6 (54.5%) in joint form of LB. Among 97 positive amplifications the most frequent target was found in primer corresponding with 16S rDNA (50 samples, 51.5%). Lower but v

Czech name

Vyšetření specifické DNA pomocí PCR u pacientů s různými formami lymeské borreliózy

Czech description

Byla zkoumána specifická DNA borrelií ve skupině 62 pacientů s různými formami lymeské borreliózy (32 pacientů s neuroboreliózou, 19 s manifestující erythema migrans a 11 s kloubními potížemi

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FE - Other fields of internal medicine

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Dermatology

ISSN

0011-9059

e-ISSN

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Volume of the periodical

47

Issue of the periodical within the volume

10

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

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UT code for WoS article

000259525000005

EID of the result in the Scopus database

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