Feasibility of fetal-derived hypermethylated RASSF1A sequence quantification in maternal plasma - Next step toward reliable non-invasive prenatal diagnostics

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F10%3A00002619" target="_blank" >RIV/00216208:11120/10:00002619 - isvavai.cz</a>

Alternative codes found

RIV/00064203:_____/10:6349

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Feasibility of fetal-derived hypermethylated RASSF1A sequence quantification in maternal plasma - Next step toward reliable non-invasive prenatal diagnostics

Original language description

We determined the feasibility of universal fetal marker detection in maternal circulation. Using real-time PCR, we compared the levels of fetal (SRY and hypermethylated RASSF1A) and total (GLO gene and total RASSF1A) extracellular DNA and fractions of extracellular fetal DNA (SRY/GLO vs. hypermethylated RASSF1A/total RASSF1A) in maternal circulation. Sensitivity and specificity reached 100% as the fetal-specific hypermethylated RASSF1A sequence was detected in all 151 examined plasma samples derived from 70 normal pregnancies with a singleton male (n = 51) or female (n = 19) fetus sampled throughout gestation and absent in non-pregnant individuals (n = 29). A strong positive correlation was observed between fetal-derived hypermethylated RASSF1A and SRY(? = 0.66, P < 0.001), total RASSF1A and GLO (? = 0.65,P < 0.001), SRY/GLO vs. hypermethylated RASSF1A/total RASSF1A ratio (? = 0.62, P < 0.001) in maternal plasma. The results indicate that a universal fetal marker could be usef

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Experimental and Molecular Pathology

ISSN

0014-4800

e-ISSN

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Volume of the periodical

89

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

7

Pages from-to

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UT code for WoS article

000284683300007

EID of the result in the Scopus database

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