A Rapid Imageable In Vivo Metastasis Assay for Circulating Tumor Cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F11%3A00003424" target="_blank" >RIV/00216208:11120/11:00003424 - isvavai.cz</a>
Result on the web
<a href="http://ar.iiarjournals.org/content/31/10/3125.full" target="_blank" >http://ar.iiarjournals.org/content/31/10/3125.full</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
A Rapid Imageable In Vivo Metastasis Assay for Circulating Tumor Cells
Original language description
Circulating tumor cells (CTCs) are of great importance for cancer diagnosis, prognosis and treatment. It is necessary to improve the ability to image and analyze them for their biological properties which determine their behavior in the patient. In the present study, using immunomagnetic beads, CTCs were rapidly isolated from the circulation of mice orthotopically implanted with human PC-3 prostate cancer cells stably expressing green fluorescent protein (GFP). The PC-3 GFP CTCs were then expanded in culture in parallel with the parental PC-3 GFP cell line. Both cell types were then inoculated onto the chorioallentoic membrane (CAM) of chick embryos. Eight days later, embryos were harvested and the brains were processed for frozen sections. The IV-100intravital laser scanning microscope enabled rapid identification of fluorescent metastatic foci within the chick embryonic brain. Inoculation of embryos with PC-3 GFP CTCs resulted in a 3 to 10-fold increase in brain metastasis when comp
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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