Differential impact of stress on hypothalamic-pituitary-adrenal axis: Gene expression changes in Lewis and Fisher rats

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43909303" target="_blank" >RIV/00216208:11120/15:43909303 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/15:00445083 RIV/00216208:11310/15:10297863

Result on the web

<a href="http://dx.doi.org/10.1016/j.psyneuen.2014.12.013" target="_blank" >http://dx.doi.org/10.1016/j.psyneuen.2014.12.013</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.psyneuen.2014.12.013" target="_blank" >10.1016/j.psyneuen.2014.12.013</a>

Result language

angličtina

Original language name

Differential impact of stress on hypothalamic-pituitary-adrenal axis: Gene expression changes in Lewis and Fisher rats

Original language description

The aim of the present work was to study the influence of variable stress on the expression of 11β-hydroxysteroid dehydrogenase type 1 (11HSD1) and the neuropeptides corticotropin-releasing hormone (CRH), urocortins 2 and 3(UCN2, UCN3), arginine vasopressin (AVP), oxytocin (OXT) and adenylate cyclase-activating polypeptide (PACAP) in two inbred rat strains: stress hypo-responsive Lewis (LEW) and hyper-responsive Fisher 344 (F344) rats. We found site-specific and strain-dependent differences in the basal and stress-stimulated expression of 11HSD1, CRH, UCN2, UCN3 and PACAP. In LEW rats, stress upregulated 11HSD1 in the prefrontal cortex and lateral amygdala, whereas in F344 rats 11HSD1 was upregulated in the central amygdala and hippocampal CA2 and ventral but not dorsal CA1 region; no effect was observed in the paraventricular nucleus, pituitary gland and adrenal cortex of both strains. The expression of glucocorticoid receptors did not parallel the upregulation of 11HSD1. Stress also stimulated the expression of paraventricular OXT, CRH, UCN3 and PACAP in both strains but amygdalar CRH only in LEW and UCN2/UCN3 in F344 rats, respectively. The upregulation of PACAP and CRH was paralleled only by increased expression of PACAP receptor PAC1 but not CRH receptor type 1. These observations provide evidence that inbred F344 and LEW rats exhibit not only the well-known phenotypic differences in the activity of the HPA axis but also strain- and stress-dependent differences in the expression of genes encoding 11HSD1 and neuropeptides associated with the HPA axis activity. Moreover, the differences in 11HSD1 expression suggest different local concentration of corticosterone and access to GR in canonical and noncanonical structures of the HPA axis.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

<a href="/en/project/GAP303%2F10%2F0969" target="_blank" >GAP303/10/0969: Local metabolism of glucocorticoids and neuroendocrine regulatory pathways during stress</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Psychoneuroendocrinology

ISSN

0306-4530

e-ISSN

—

Volume of the periodical

53

Issue of the periodical within the volume

March

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

49-59

UT code for WoS article

000350518000006

EID of the result in the Scopus database

2-s2.0-84922764413