Assessing the function of mitochondria in cytosolic context in human skeletal muscle: Adopting high-resolution respirometry to homogenate of needle biopsy tissue samples
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43909397" target="_blank" >RIV/00216208:11120/15:43909397 - isvavai.cz</a>
Alternative codes found
RIV/00064173:_____/15:#0000447
Result on the web
<a href="http://dx.doi.org/10.1016/j.mito.2015.02.002" target="_blank" >http://dx.doi.org/10.1016/j.mito.2015.02.002</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.mito.2015.02.002" target="_blank" >10.1016/j.mito.2015.02.002</a>
Alternative languages
Result language
angličtina
Original language name
Assessing the function of mitochondria in cytosolic context in human skeletal muscle: Adopting high-resolution respirometry to homogenate of needle biopsy tissue samples
Original language description
Using skeletal muscle homogenates for respirometry has many advantages, but the main challenge is avoiding the damage to outer mitochondrial membrane (OMM) and complex I. By optimising the amount of muscle and careful titration of substrates and inhibitors we developed a new protocol and compared it to isolated mitochondria. We found acceptable damage to OMM (~10-15% increment of oxygen flux after addition of cytochrome c) and to complex I (~70% of electron flux). Homogenate retained ~90% of phosphorylation capacity of isolated mitochondria. The use of fresh homogenate was crucial as mitochondrial function declined rapidly after 2-3h of cold storage.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/NT12319" target="_blank" >NT12319: Multiparameter metabolic analysis of human myoblasts related to the pathogenesis of intensive care unit acquired weakness</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Mitochondrion
ISSN
1567-7249
e-ISSN
1872-8278
Volume of the periodical
21
Issue of the periodical within the volume
March
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
106-112
UT code for WoS article
000351328700013
EID of the result in the Scopus database
2-s2.0-84923080728