Development and validation of LC-MS/MS method for quantification of bisphenol A and estrogens in human plasma and seminal fluid
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43909811" target="_blank" >RIV/00216208:11120/15:43909811 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/15:10312390 RIV/00023761:_____/15:#0000542 RIV/00064173:_____/15:#0000463
Result on the web
<a href="http://dx.doi.org/10.1016/j.talanta.2015.03.013" target="_blank" >http://dx.doi.org/10.1016/j.talanta.2015.03.013</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.talanta.2015.03.013" target="_blank" >10.1016/j.talanta.2015.03.013</a>
Alternative languages
Result language
angličtina
Original language name
Development and validation of LC-MS/MS method for quantification of bisphenol A and estrogens in human plasma and seminal fluid
Original language description
Bisphenol A (BPA) is a widely known endocrine disruptor with estrogenic, antiestrogenic or antiandrogenic properties. BPA could interfere with estrogen metabolism as well with receptor-mediated estrogen actions. Both environmental BPA and estrogens may be traced in body fluids, of which, besides the blood plasma, the seminal fluid is of particular interest regarding their possible interactions in the testis. The method for simultaneously determining BPA and estrogens is then needed, taking into account that their concentrations in these body fluid may differ. Here the method was developed and validated for measurements of BPA, estrone (E1), estradiol (E2) and estriol (E3) in blood plasma and seminal plasma using liquid chromatography-tandem mass spectrometry. Due to the phenolic moiety of all compounds, dansyl chloride derivatization could be used. The analytical criteria of the method with respect to expected concentration of the analytes were satisfactory. The lower limits of quantifications (LLOQ) amounted to 43.5, 4.0, 12.7, 6.7pg/mL for plasma BPA, E1, E2 and E3, and 28.9, 4.9, 4.5, 3.4pg/mL for seminal BPA, E1, E2 and E3, respectively. The concentrations of individual steroids differed between body fluids. To the best of our knowledge, this is the first method that enabled the measurement of estrogens and BPA together in one run. The concentrations of E1, E2 and for the first time also of E3 in seminal plasma in normospermic men are reported.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FB - Endocrinology, diabetology, metabolism, nutrition
OECD FORD branch
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Result continuities
Project
<a href="/en/project/NT13369" target="_blank" >NT13369: The impact of selected endocrine disruptors on human spermatogenesis</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Talanta
ISSN
0039-9140
e-ISSN
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Volume of the periodical
140
Issue of the periodical within the volume
August
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
62-67
UT code for WoS article
000356987400010
EID of the result in the Scopus database
2-s2.0-84925679903