Small nucleolar RNA U91 is a new internal control for accurate microRNAs quantification in pancreatic cancer

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43910359" target="_blank" >RIV/00216208:11120/15:43910359 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1186/s12885-015-1785-9" target="_blank" >http://dx.doi.org/10.1186/s12885-015-1785-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12885-015-1785-9" target="_blank" >10.1186/s12885-015-1785-9</a>

Result language

angličtina

Original language name

Small nucleolar RNA U91 is a new internal control for accurate microRNAs quantification in pancreatic cancer

Original language description

BACKGROUND: RT-qPCR quantification of miRNAs expression may play an essential role in pancreatic ductal adenocarcinoma (PDAC) diagnostics. RT-qPCR-based experiments require endogenous controls for the result normalization and reliability. However, expression instability of reference genes in tumors may introduce bias when determining miRNA levels. METHODS: We investigated expression of 6 miRNAs, isolated from FFPE samples of pancreatic adenocarcinomas. Four internal controls were utilized for RT-qPCR result normalization: artificial miR-39 from C. elegans, U6 snRNA, miR-16 and snoRNA U91. RESULTS: We found miR-21, miR-155 or miR-217 expression values in tumors may differ up to several times, depending on selected internal controls. Moreover, differentinternal controls can produce controversial results for miR-96, miR-148a or miR-196a quantification. Also, expression of our endogenous controls varied significantly in tumors. U6 demonstrated variation from -1.03 to 8.12-fold, miR-16 fro

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC Cancer

ISSN

1471-2407

e-ISSN

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Volume of the periodical

15

Issue of the periodical within the volume

-

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

"Article 774"

UT code for WoS article

000363376700004

EID of the result in the Scopus database

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