The use of polarity switching for the sensitive determination of nitrate in human cerebrospinal fluid by capillary electrophoresis with contactless conductivity detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F16%3A43911348" target="_blank" >RIV/00216208:11120/16:43911348 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.chroma.2016.04.038" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2016.04.038</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2016.04.038" target="_blank" >10.1016/j.chroma.2016.04.038</a>

Result language

angličtina

Original language name

The use of polarity switching for the sensitive determination of nitrate in human cerebrospinal fluid by capillary electrophoresis with contactless conductivity detection

Original language description

A new electrophoretic stacking method has been developed for the sensitive determination of nitrates in cerebrospinal fluid. 2M acetic acid was used as the BGE; inorganic anions were detected using a contactless conductivity detector and separation was carried out in an INST-coated capillary with inner diameter of 25μm. The sample of cerebrospinal fluid was injected in a large volume into the short end of the separation capillary (15cm) and separation first occurred in the isotachophoretic mode, where a long zone of the majority chloride migrates in the capillary and is followed by a concentrated zone of the unseparated nitrates. The sample zone passes to the end of the capillary where more than 99% of the chlorides are let out. Then the polarity of the voltage is switched and separation occurs in the zone electrophoresis mode, in which the nitrates are separated from the zone of chlorides. The time of switching the polarity is determined by the decrease in the electrophoretic current. Up to 99.95% of the original amount of chlorides present in the cerebrospinal fluid could be let out of the capillary by this technique, thus increasing the signal/noise ratio by up to 60-fold compared to classical electrophoretic separation.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA15-03139S" target="_blank" >GA15-03139S: New Electrophoretic Approaches in Studies of Obesity and Diabetes</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Volume of the periodical

1447

Issue of the periodical within the volume

May

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

148-154

UT code for WoS article

000375888600018

EID of the result in the Scopus database

2-s2.0-84981764243