Hydrodynamic sample injection into short electrophoretic capillary in systems with a flow-gating interface

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F17%3A43912636" target="_blank" >RIV/00216208:11120/17:43912636 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/17:10359494
Result on the web
<a href="http://dx.doi.org/10.1016/j.chroma.2016.12.029" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2016.12.029</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2016.12.029" target="_blank" >10.1016/j.chroma.2016.12.029</a>

Result language
angličtina
Original language name
Hydrodynamic sample injection into short electrophoretic capillary in systems with a flow-gating interface
Original language description
An electrophoretic apparatus with a flow-gating interface has been developed, enabling hydrodynamic sequence injection of the sample into the separation capillary from the liquid flow by underpressure generated in the outlet electrophoretic vessel. The properties of the apparatus were tested on an artificial sample of an equimolar mixture of 100μM potassium and sodium ions and arginine. The repeatability of the injection of the tested ions expressed as RSD (in%) for the peak area, peak height and migration time was in the range 0.76-2.08, 0.18-0.68 and 0.28-0.48, respectively. Under optimum conditions, the apparatus was used for sequence monitoring of the reaction between the antidiabetic drug phenyl biguanide and the glycation agent methyl glyoxal. The reaction solution was continuously sampled by a microdialysis probe from a thermostated external vessel using a syringe pump at a flow rate of 3μLmin(-1) and was injected into a separation capillary at certain time intervals. The electrophoretic separation progressed in a capillary with an internal diameter of 50μm with a length of 11.5cm and was monitored using a contactless conductivity detector..
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry

Project
<a href="/en/project/GA15-03139S" target="_blank" >GA15-03139S: New Electrophoretic Approaches in Studies of Obesity and Diabetes</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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