Ultrasound-assisted production of highly-purified β-glucan schizophyllan and characterization of its immune properties
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F17%3A43913190" target="_blank" >RIV/00216208:11120/17:43913190 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.procbio.2017.04.016" target="_blank" >http://dx.doi.org/10.1016/j.procbio.2017.04.016</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.procbio.2017.04.016" target="_blank" >10.1016/j.procbio.2017.04.016</a>
Alternative languages
Result language
angličtina
Original language name
Ultrasound-assisted production of highly-purified β-glucan schizophyllan and characterization of its immune properties
Original language description
In this work, we suggest procedure for manufacturing of highly pure schizophyllan (SPG) cleaved into shorter fragments by ultrasonication. The ultrasonicated SPG (uSPG) was prepared from Schizophyllum commune submerged cultivation in bioreactor, culture broth ultrasonication with 100. Ws/mL energy input, followed by depth filtration and ultrafiltration. The uSPG contained less than 0.3%w/w protein and 0.078. IU/mg endotoxin. Chemical composition and structure of the uSPG were analyzed by GC and NMR, and conformation of the molecules was documented by AFM. Apyrogenicity of uSPG was proved by MAT test. Immunomodulatory properties of native and denatured uSPG were studied in vitro with human whole blood. The LPS-spiked samples revealed immunomodulatory activity of both native and denatured uSPG. Protein array showed that native uSPG activates certain cytokines and chemokines significantly stronger than denatured uSPG.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Process Biochemistry
ISSN
1359-5113
e-ISSN
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Volume of the periodical
58
Issue of the periodical within the volume
July
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
313-319
UT code for WoS article
000404710700042
EID of the result in the Scopus database
2-s2.0-85018570879